ISSN:
1573-2576
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Gossypol is present in antiinflammatory poultices made from the medicinal treeThespesia populnea. Isolated human neutrophils exposed to 3–20μM gossypol for 15–90 min were assayed in vitro for superoxide production and surface expression of Mac-1 (CD11b/CD18). Gossypol increased superoxide production in a time- and concentration-dependent fashion consistent with a moderate, delayed respiratory burst. Surface Mac-1 expression was increased within 15 min by 3–5μ M gossypol, resulting in a 14-fold increase over controls and a threefold greater increase over that produced by PMA. Staurosporine failed to block gossypol induction of superoxide and Mac-1, while EDTA inhibited induction of Mac-1 only, implicating a calcium-dependent mechanism. Gossypol increased intracellular calcium to peak levels, but1 in a delayed fashion as compared to FMLP. These findings demonstrate that gossypol is a highly potent stimulant of Mac-1 expression and suggest at least two protein kinase C-independent pathways of neutrophil activation. The resultant exhaustion of neutrophils may account for the antiinflammatory properties of plants containing gossypol.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01560692
