ISSN:
1573-7330
Keywords:
embryo culture
;
density
;
culture volume
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Description / Table of Contents:
Methods Embryos were collected from immature C57BL6 female mice superovulated with pregnant mare serum gonadotropin and human chorionic gonadotropin and mated by CD1 males. Groups of 1, 5, 10, or 20 embryos were cultured in 5-, 10-, 20-, or 40-µl drops of CZB under silicon oil at 37.5°C in a humidified atmosphere of 5% CO 2 and 95% air.
Abstract:
Results Development score for embryos cultured in 10 µl was higher than that of embryos cultured in 20 or 40 µl. Embryos cultured in groups of 5, 10, or 20 had higher development scores than embryos cultured singly. The highest development score was obtained by the combination of 5 embryos per 10-µl drop. The percentage of live embryos in 20 or 40 µl was lower than that of embryos cultured in 10 µl. Additionally, the percentage of live embryos cultured singly was lower than that of embryos cultured in groups.
Notes:
Background One-cell mouse embryos were used to determine the effects of drop size and number of embryos per drop for optimum development in vitro.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01204051
