ISSN:
1573-7373
Keywords:
mitoxantrone
;
malignant human brain tumors
;
DNA strand breaks
;
alkaline elution
;
flourescence spectrophotometry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract In this study mitoxantrone (Mtx) induced DNA strand breaks were measured with the alkaline elution technique in short term cell cultures derived from human gliomas. Glioblastomas or astrocytomasfrom 5 patients who underwent intracranial surgery were cultured and incubated 1 h with different concentrations of Mtx (0, 0.01, 0.1 and 1.0 μg/ml). The alkaline elution methodwas modified to measure DNA lesions in human gliomas. Mtx inducedDNA strand breaks in a dose dependent manner in all cell culturestested. There was a linear increase of DNA strand break frequencyinduced by Mtx between 0.01—1.0 μg/ml. Concerning these in vitro data, Mtx might be potentially useful for the treatment ofpatients with malignant brain tumors.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005792410604
