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Digitale Medien

Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol (1976)

Davidson, Richard L. ; Gerald, Park S.

Springer

Somatic cell and molecular genetics 2 (1976), S. 165-176

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Details

ISSN: 1572-9931

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract Modifications in the techniques for the induction of mammalian somatic cell hybridization by polyethylene glycol (PEG) have led to procedures that are rapid, simple, and effective. The basic improvements, for both monolayer and suspension fusions, are a short exposure to PEG and a rapid dilution of PEG following treatment. There is a marked effect of PEG concentration on cell hybridization, and there seem to be inherent differences between cells in terms of the extent of cell fusion induced by PEG.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01542629

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