ISSN:
1432-0428
Keywords:
Insulin
;
receptors
;
mouse embryo retina
;
Simian virus 40
;
established cell lines
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The binding of125I-insulin to uncloned and cloned cultures of mouse retinal cells has been investigated. At 15° C, binding of the hormone reached a steady state by 60 min, while at 37° C equilibrium was reached earlier but at a lower level than at 15° C. Porcine insulin, porcine proinsulin and guinea pig insulin displaced labelled insulin in proportion to their known biological potency. A sharp pH dependence of the hormone binding was observed with an optimum at pH 7.8. The dissociation rate of the125I-insulin was increased in the presence of unlabelled hormone, suggesting the existence of negative cooperativity in the insulin-receptor interaction. The availability of established retinal cell lines with insulin receptors should facilitate the study of the insulin-retina interactions in a controlled in vitro system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01221955
