ISSN:
1432-072X
Keywords:
α Pheromone
;
Cycloheximide
;
Inducible a strain
;
Phenylmethylsulfonyl fluoride
;
Saccharomyces cerevisiae
;
Sexual agglutinability
;
Temperature-sensitive
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract When α pheromone-pretreated cells of an inducible a strain of Saccharomyces cerevisiae carrying the inducible gene saa1 were incubated in a growth medium at 28°C, induction of sexual agglutinability began after a 10 min lag period. If the cells were incubated at 38°C during the lag period, no induction occurred even after incubation at 28°C. Contrary to this, if the cells were incubated at 28°C during the lag period, almost complete induction occurred, even after transfer to 38°C. Temperature shift experiments revealed that 5 min incubation at 28°C was necessary for the initiation of the temperature-sensitive period and further 5 min incubation for the completion of the period. The temperature-sensitive period was sensitive to phenylmethylsulfonyl fluoride.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00407957
