ISSN:
1432-072X
Keywords:
Neurospora crassa ‘Slime” variant
;
Cell coat
;
Glycoproteins
;
Self-assembling
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The proteinaceous extracellular material (PEM) synthesized by the cells of the ‘slime” strain of Neurospora crassa (see Martinez et al. 1989) was solubilized by treatment with urea or guanidine. Removal of these chemicals by dialysis, caused reassembly of the solubilized proteins into material with the same microscopic appearance as the original PEM. Polypeptide patterns from both native and reassembled structures were identical. Dialysis-mediated reassembly of the solubilized proteins appeared to be dependent on both concentration of the soluble macromolecules and time. Gel chromatography of PEM solubilized with different agents revealed two discrete populations of complexes with molecular masses of 1,500 and 500 kDa respectively. These were able to reassemble into lamellar structures with a variable degree of efficiency.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00447008
