ISSN:
1432-072X
Keywords:
Nitrate photoassimilation
;
Nitrite
;
Fumarase
;
Aconitase
;
Rhodobacter capsulatus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract High levels of aconitase and fumarase activities were found in Rhodobacter capsulatus E1F1 cells cultured with nitrate as the sole nitrogen source either under light-anaerobic or dark-aerobic conditions. Both activities were strongly and reversibly inhibited in vitro by nitrite or nitric oxide, whereas nitrate or hydroxylamine showed a lower effect. Other enzymes of the tricarboxylic acids cycle such as malate dehydrogenase or isocitrate dehydrogenase were not affected by these nitrogen compounds. When growing on nitrate in the dark R. capsulatus E1F1 cells accumulated nitrite intracellularly, so that an in vivo inhibition of aconitase and fumarase could account for the strong inhibition of growth observed in the presence of nitrite under dark-aerobic conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00248609
