ISSN:
1432-072X
Keywords:
Methylenetetrahydrofolate
;
Methyltetrahydrofolate
;
Methylenetetrahydrofolate reductase
;
Homoacetogenic bacteria
;
Energy conservation of homoacetogens
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The thermodynamics of the methylenetetrahydrofolate reduction to 5-methyltetrahydrofolate was studied with the methylenetetrahydrofolate reductase purified from the homoacetogenic bacterium Peptostreptococcus productus. The equilibrium constants were determined for the forward and backward reactions of methylenetetrahydrofolate reduction with NADH or acetylpyridine adenine dinucleotide (APADH), respectively, as the electron donors. From the equilibrium constants and the known standard redox potentials at pH 7 (E o′ ) of the couples NAD+/NADH or APAD+/APADH the E o′ of the couple methylene-/methyltetrahydrofolate was determined to be about-200mV. This value is different from values reported before for this couple. The implications for the mechanism of energy conservation of homoacetogens is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00243458
