ISSN:
1432-1912
Keywords:
Dopamine
;
DES
;
Adenosine
;
cAMP
;
Hypothalamus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Dopamine (DA) stimulates the cAMP-generating system in the male rat hypothalamus only to a very low extent (25% above control). Diethylstilbestrol (DES), a synthetic estrogen, was found to be extremely potent (a 4- and 16-fold stimulation at 20 μM and 100 μM, respectively). Addition of either one to an incubation medium containing varying concentrations of the other resulted in a synergistic response. The potentiation by 20 μM DES of the effect elicited by 100 μM DA was the most remarkable, namely, a 3-fold stimulation of the combined response. A 4- and 7.5-fold stimulation of cAMP accumulation was observed when adenosine (100 μM) or adenosine (100 μM)+DA (100 μM) were present in the incubation medium. Theophylline (0.5 mM), an adenosine antagonist, could effectively reduce this effect, as did adenosine deaminase (10 μg/ml). Clomiphene (50 μM), an estrogen antagonist, exhibited a marked decrease in DES+DA-elicited cAMP formation. Pimozide (40 μM) had the ability to significantly block the stimulatory effects of DES and DA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00508300
