Electronic Resource
Springer
Naunyn-Schmiedeberg's archives of pharmacology
317 (1981), S. 61-66
ISSN:
1432-1912
Keywords:
GABA
;
Rat
;
Thyroid gland
;
Transport
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary 1. The uptake of γ-aminobutyric acid (GABA) into rat thyroid slices was studied. 2. Uptake of 14C-GABA was concentration-dependent: one unsaturable (diffusion) and two saturable components obeying Michaelis-Menten kinetics contributed to transport. 3. The kinetic constants of saturable GABA transport systems were: K m1 =1.5 μM, V 1=4.0 nmol×(g wet weight)−1×(20 min)−1 (high-affinity uptake); K m2 =800 μM, V 2=260 nmol×(g wet weight)−1×(20 min)−1 (low-affinity uptake). 4. Uptake mediated by each of the carrier systems was concentrative, entirely Na+-dependent, and required activation energies characteristic for active transport. 5. High-affinity transport was structurally specific for GABA. The substrate specificity of low-affinity uptake resembled that of β-amino acid transport systems.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00506258
| Library |
Location |
Call Number |
Volume/Issue/Year |
Availability |
