ISSN:
1432-2013
Keywords:
Renin
;
Juxtaglomerular Apparatus
;
Rat Renin Substrate
;
Angiotensinase
;
Renin
;
Juxtaglomerulärer Apparat
;
Ratten-Reninsubstrat
;
Angiotensinase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary We have established a method for the determination of renin activity in minute amounts of tissue. This method was used for single juxtaglomerular apparatuses (JGA's) of rats. Enzyme kinetic studies were performed to establish the optimal conditions for microdissected JGA's, using purified rat renin and its substrate. Maximal formation of angiotensin occurs at pH 6.5. The effect of incubation and homogenization time, temperature, substrate and renin concentration on the angiotensin formation rate was studied. For the studies reported here purified rat renin substrate was used. The purification methods revealed a substrate content of appr. 1000 ng/mg protein. This highly purified rat renin substrate improved the sensitivity of renin determination in micro amounts of tissue. Hence renin activity of single microdissected JGA's could be quantitatively determined within 1 hour of incubation in a volume of 0.1 ml. Mean renin activity in 18 JGA's from rats kept on standard Altromin diet was 15.2±S.D. 7.0 ng/0.1 ml·hour.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00588645
