ISSN:
1432-2013
Keywords:
Phloretin
;
Frog skin
;
Urea transport
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract This report presents evidence for urea active absorption by isolated skin of Rana esculenta. One of the supporting factors of such evidence is that at a low concentration the urea influx is five times greater than the outflux, in the absence of a chemical gradient. The transport shows a saturation kinetics with an apparentK m =1.33mM and is inhibited by an uncoupling agent (ECCP). 5·10−4M Phloretin, added to the external side, markedly inhibits inward urea transport, whereas it is ineffective when added to the serosal fluid. This provides evidence for a phloretin-sensitive mechanism located at the external side of the epithelium. Phloretin stimulates the sodium active transport; the possible coupling of urea and sodium movement is analysed
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00589096
