ISSN:
1432-2013
Keywords:
Key-words: Vascular smooth muscle, Calcium signalling, Development, L-type Calcium Channels, Nitrendipine binding sites
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract We have investigated whole-cell Ba2+ currents through Ca2+ channels (IBa) in single myocytes freshly isolated from the aortic media of neonatal (1-day-old) and adult (12-week-old) rats. In neonatal myocytes, (IBa) was undetectable even in presence of the dihydropyridine (DHP) agonist Bay K 8644. Binding of [3H]Nitrendipine on crude plasma membrane preparation of media confirmed the absence of DHP-receptors. By contrast, a robust DHP-sensitive 'L-type' IBa was recorded in adults which was consistent with the presence of specific [3H]Nitrendipine binding sites. In conclusion, neonatal aortic myocytes do not express any Ca2+ channels. The acquisition of L-type Ca2+ channels may be related to cell differentiation and acquisition of contractility during postnatal development.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02253845
