ISSN:
1432-2013
Keywords:
Key words Cardiac myocytes
;
Excitation-contraction coupling
;
Sarcomere length
;
Twitch contraction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract An improved method was developed for measuring sarcomere length (SML) during twitch contractions of single cardiac ventricular myocytes, using a charge-coupled photodiode array self-scanning at a rate of 1.5 ms/element. The average resting SML of 111 cells was 1.88±0.04 µm (mean ±SD). When contractions were triggered by action potentials under perforated-patch conditions, the time course of SML shortening closely followed changes in cell length. A large variation was observed in contraction time course between myocytes, some cells having a phasic component with a duration at 50% shortening (full-width at half-maximum; FWHM) of approximately 40 ms, while others shortened more slowly (FWHM of phasic component @100 ms). FWHM was highly correlated with relaxation half-time, but with neither action potential duration nor resting SML. The kinetics of slowly contracting cells could not be converted to the rapid type by using conditioning trains or applying isoprenaline. The steady-state SML/pCa relation in ventricular myocytes was measured by applying solutions of various pCa immediately after localized punctures of the surface membrane using a focal laser beam. The Hill coefficient, n H, was @4–5 and K 1/2@400–500 nM, but there was no evidence of two populations of cells with different Ca2+ sensitivities.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004240050849
