ISSN:
1432-0797
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract The batch productivity (Q TM) of the production of the nucleoside antibiotic toyocamycin (TM) by Streptomyces chrestomyceticus was increased ten-fold by selection of a UV generated mutant, optimization of pH, increasing incubation temperature from 28 °C to 36 °C, and addition of soy oil. Initial high oxygen transfer rates stimulated Q TM maxima two-fold. Antibiotic production by the mutant strain, U190, however, appeared more shear sensitive than the parent culture FCRF 341 with maximum antibiotic titer being inversely related to impellor tip velocity, T v . For this reason, scale-up could not be done at constant P/V or constant volumetric oxygen transfer. Instead, programming of impeller speed was evaluated in order to maintain optimal impeller tip velocity during scale-up. It was found that a low constant T v maintained in scale-up in geometrically similar vessels was most beneficial for duplication of optimal antibiotic productivity, Q TM. Pilot fermentations (120 dm3 scale) were used to determine coefficients of Q TM variation from oxygen uptake rate (OUR) and total CO2 evolution data for monitoring of Q TM variation during scale-up to the 12,000 dm3 scale. This technique allowed for on-line prediction of antibiotic titer and Q TM from fermentor exhaust gas data.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00369578
