ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract During the last 5 years, strain improvement by genetic engineering has been established as a good alternative for traditional methods of strain improvement such as mutagenesis and genetic recombination. Considerable success has been achieved in the overproduction of a variety of fungal proteins. However, from the available data a limitation at the level of transcription is evident. A more detailed analysis of this transcription limitation was carried out for the overproduction of glucoamylase in A. niger. This analysis revealed that both the site of integration of introduced gene copies and the available amount of trans-acting regulatory proteins were at the root of the observed limitation. Based on these results, approaches for the construction of a new generation of protein-overproducing strains are suggested.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002530050390
