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Detection of ferulic acid esterase production by Bacillus spp. and lactobacilli (1998)

Donaghy, J. ; Kelly, P. F. ; McKay, A. M.

Springer

Applied microbiology and biotechnology 50 (1998), S. 257-260

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ISSN: 1432-0614

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract The production of feruloyl esterase activity by Bacillus spp. and lactobacilli can be detected in an agar-plate assay. The assay involves the substitution of the main carbon source in specific agar with ethyl ferulate. A number of Bacillus spp., predominantly B. subtilis strains, were found to exhibit feruloyl esterase activity by this method. Of the examined lactobacilli, Lb. fermentum (NCFB 1751) showed the highest level of ferulic acid esterase activity. The enzyme was released from harvested cells by sonication and showed pH and temperature optima of 6.5 and 30 °C respectively.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s002530051286

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