ISSN:
1432-0584
Keywords:
Immunoglobulin expression
;
μ-mRNA
;
In situ hybridization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Cells from a normal donor, a B-CLL and a T-ALL were labeled with an FITC conjugated rabbit antihuman IgM and hybridized with a rhodamineconjugated cloned DNA coding for the cμ segment. The cells were measured simultaneously by immunofluorimetry for their surface content of IgM and their amount of m-RNA hybridizing with the cloned DNA. Thus it was possible to compare the transcription of the gene coding for the immunoglobulin μ chain and the expression of IgM in individual cells. It could be shown that besides the immunoglobulin μ containing B-cells, which were expected to contain the respective m-RNA, cells were found which did not express IgM but which were still positive for the m-RNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00320667