ISSN:
1432-0843
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The intracellular metabolism of daunorubicin (DNR) has been studied in sensitive and resistant Ehrlich ascites tumor (EAT) cells. The subcellular localization of metabolites has been followed by normal-phase and reverse-phase high-pressure liquid chromatography (HPLC). The metabolism of DNR by either sensitive or resistant EAT cells is not significant; unmetabolized DNR is always the main intracellular compound. Daunorubicinol (DOL) accounts for less than 5% after 24h and an unidentified product is also observed. This highly apolar compound, having an intrinsic fluorescence one order of magnitude greater than that of DNR is formed in acellular conditions and could be a chemical artifact. DNR and DOL are mainly associated with DNA-containing fractions. No significant differences can be observed in the metabolism of DNR in sensitive and resistant EAT cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00296761
