ISSN:
1432-0983
Keywords:
Polyadenylation
;
RNA 3′-end formation
;
Transcription termination
;
Yeast
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have tested the functioning of the human c-myc polyadenylation signal in Saccharomyces cerevisiae. A DNA fragment containing the two AATAAA polyadenylation signals of the c-myc gene was inserted into a plasmid designed for the in-vivo testing of polyadenylation signals in yeast. The c-myc fragment had a partial capacity for directing mRNA 3′-end formation in yeast. The 3′-endpoints were 50–100 bp distant from the mRNA 3′-ends mapped in humans. This human DNA fragment is therefore unspecifically functional in yeast, indicating that other sequence elements than the human polyadenylation signal, AATAAA, are necessary for 3′-end formation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00351496
