ISSN:
1432-0983
Keywords:
Neurospora crassa
;
bli-7, eas, ccg-2
;
Promoter analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Expression of the Neurospora crassa gene bli-7, (identical with eas, and ccg-2), is induced by blue light, as well as glucose- or nitrogen-starvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of foreign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducibility of the gene, in response to the three inducing conditions mentioned, is lost by eliminating the region between-1498 bp and-1017 bp upstream of the transcription start point. A segment with an apparently negative effect was found between-595 bp and-429 bp, as well as a stretch of DNA from-429 bp to-380 bp which may exert a positive influence after light induction.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00351847
