Electronic Resource
Springer
Plant cell reports
12 (1993), S. 180-183
ISSN:
1432-203X
Keywords:
Peas
;
Seed
;
Transformation
;
Agrobacterium
;
Meristems
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The lateral cotyledonary meristems present in germinating seed were inoculated with a non-oncogenic strain of A. tumefaciens carrying a gene conferring kanamycin resistance as a selectable marker and a β-glucuronidase sequence as a reporter gene. Kanamycin resistant plants were derived from the meristems and shown to be transformed on the basis of Southern blots, polymerase chain reaction analysis and tests for β-glucuronidase activity. The plants were fertile and tests of their progeny confirmed the transmission of integrated sequences through a sexual generation. This transformation method has the merit of an unlimited supply of material for inoculation and a relatively short time scale from inoculation to the production of rooted plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00239102
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