ISSN:
1432-203X
Keywords:
protoplast culture
;
plant regeneration
;
somatic embryogenesis
;
Hordeum murinum L.
;
wild resource
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have produced a large number of plants regenerated from protoplasts originally isolated from embryo-derived cell suspensions of wild barley, Hordeum murinum L.. Suspensions initially allowed protoplast isolation and culture 5.5 to 9 months from the date of callus initiation. Colony formation efficiencies ranged from 1.5 to 3.0 % and from 0.1 to 1.4 % for protoplast cultures with and without nurse cells, respectively. Both nurse and non-nurse techniques allowed efficient embryogenesis and plant regeneration. More than 400 shoots/plantlets have been obtained from 6 independent experiments. Over 150 plants have been transferred to the greenhouse. Protoplasts isolated from the youngest suspensions (5.5 months old) gave rise to the largest number of plants. Protoplasts isolated from suspensions as old as 15 months were also regenerable.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00239880
