ISSN:
1432-203X
Keywords:
Key wordsPrunus persica Somaclonal variation
;
AbbreviationsPCR Polymerase chain reaction
;
RAPD random amplified polymorphic DNA
;
RFLP Restriction fragment length polymorphism
;
CTAB cetyltrimethyl ammonium bromide
;
PVP polyvinyl pyrolidone
;
dNTP deoxy-ribonucleotide triphosphate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Peach [Prunus persica (L.) Batsch] regenerants from cv `Sunhigh' embryo no. 156, regenerants obtained from cv `Redhaven' embryo no. 30, and two peach cultivars `Sunhigh' and `Redhaven', were screened for polymorphic RAPD (Random Amplified Polymorphic DNA) markers with up to 60 10-mer primers. Although 35 primers produced results with scoreable bands, only 10 of the primers revealed polymorphism for regenerants of embryo no.156 and cv `Sunhigh', and 1 revealed a low level of polymorphism for regenerants of embryo no. 30 and cv `Redhaven'. This study demonstrates the feasibility of using RAPD markers to identify somaclonal variants of peach and provides evidence for the existence of genetic differences among these variants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050290
