ISSN:
1432-203X
Keywords:
Key words Pennisetum glaucum (L.) R.Br. (pearl millet)
;
In vitro
;
Multiple shoots
;
Meristems
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract This report presents a procedure for high-frequency multiple shoot production from cultured shoot apical meristems of pearl millet [Pennisetum glaucum (L.) R. Br.]. Shoot apices from 1-week-old aseptically germinated seedlings were cultured in vitro on MS medium containing various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BA) with biweekly subculture. A low concentration of 2,4-D coupled with four different concentrations of BA induced the production of adventitious shoots from the enlarged shoot apical meristems. Somatic embryogenesis was also observed at higher concentrations of BA. The use of higher levels of 2,4-D resulted in callusing of shoot apical meristems, while the shoot tips produced many leaves and in vitro flowering in 2,4-D-free media containing BA. All four pearl millet genotypes produced similar results. Fertile pearl millet plants were produced from in vitro-produced multiple shoots.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050771
