ISSN:
1432-203X
Keywords:
Oryza
;
Somatic embryogenesis
;
Immature panicle culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract An embryogenic callus was obtained from immature panicle of an interspecific hybrid (Oryza sativa x O. latifolia) F1. The medium consisted of HE salts supplemented with 2,4-D, NAA (each 2 mg/l), kinetin (3 mg/l), yeast extract (1360 mg/l) and casein hydrolyzate (300 mg/l). The callus was milk-white in colour compact and granulate in texture. Various developmental stage of embryoid, such as globular, heart-shape, scutellum-shape and mature embryoid were observed in an embryogenic callus. Plantlets were successfully regenerated from 1-month-old callus with more than 80% regenerational frequency in each subculture for 12 passages.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00270094
