ISSN:
1432-203X
Keywords:
sunflower
;
protoplasts
;
direct gene transfer
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Helianthus annuus protoplasts were transformed with the plasmid pCaMVNEO (Frommet al. 1986) conferring kanamycin resistance to plant. Transformed calli were selected with a frequency of 4 calli for 106 treated protoplasts. DNA was extracted from kanamycin resistant calli. Analysis of this DNA shows the presence of the NPTII gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00716848