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Plant regeneration from protoplasts of common buckwheat (fagopyrum esculentum) (1989)

Adachi, T. ; Yamaguchi, A. ; Miike, Y. ; [et al.]

Springer

Plant cell reports 8 (1989), S. 247-250

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ISSN: 1432-203X

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Protoplasts were isolated from hypocotyls of etiolated seedlings from a diploid and the corresponding autotetraploid variety of common buckwheat (Fagopyrum esculentum). The isolated protoplasts started to divide after 4 days in culture in a modified MS medium. Maximum plating efficiency was approximately 1%. Regenerated calli derived from the tetraploid genotype developed roots easily but were recalcitrant to form shoots. Eighteen months following the initiation of cultures, tetraploid embryoids and shoots emerged after 3 weeks on an MS medium containing 0.1 mg/l gibberellic acid.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00778544

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