ISSN:
1432-0789
Keywords:
Key words Most probable number (MPN)
;
Plate count
;
Bradyrhizobium japonicum
;
Nodulatingscoring
;
Soybean
;
Glycine max
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Geosciences
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract The reliability of the most probable number (MPN) method for estimating bradyrhizobial numbers was evaluated by comparison with the plate count procedure. MPN estimates increased with time of nodulation scoring after seedling inoculation through 6 weeks of incubation. Ratios of MPN to plate counts increased as the bradyrhizobial cell suspension concentration increased. The MPN method could not detect Bradyrhizobium japonicum numbers at concentrations of 103 colony forming units (CFU) ml–1 and below. A proposal for re-evaluating MPN estimates is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00336049
