ISSN:
1432-0533
Keywords:
Human Adenovirus Type 12
;
Sprague-Dawley Rats
;
Intracerebral Inoculation
;
Medulloepitheliomatous Neoplasm
;
Giant Tumor Cells
;
Electron Microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Solid medullary brain and spinal cord neoplasms developed in all 10 offspring of an outbred Sprague-Dawley rat, between 37 and 99 days after a single postnatal (within 24 hrs) inoculation of 0.05 ml of human adenovirus type 12, 103.5–104.5 TCID50 HeLa cells/0.1 ml in the left frontal lobe. Seven rats developed multicentric neoplasms in both hemispheres and in peri-aqueductal areas of the brain stem, one of which was associated with an incipient spinal cord tumor in the sacral segment. One rat developed a solid tumor involving the right parieto-occipital region. The remaining two cases were solid spinal cord tumors arising from the dorsal half of the thoracolumbar segments. The remarkably uniform microscopic appearance was designated as a counterpart of human embryonic neuronal neoplasms. Characteristic neuronal and multinucleated giant cells emerged throughout the tumor tissue with argentaffine, neurofibril-like cytoplasmic expansions and a unique cilium (a 9+0 pattern of tubules) associated with a pair of centrioles. This cilium morphology was also a hallmark of the majority of tumor cells that formed characteristic pseudorosettes. The occasional emergence of two sets of cilia and centrioles in monstrous cells suggested probable modes of cytogenesis in relation to cessation of abnormal cell division.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00697014
