ISSN:
1432-0533
Keywords:
Myxopapillary Ependymoma
;
Tissue and Organ Culture
;
Electron Microscopy
;
Basement Membrane
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Explants of a myxopapillary ependymoma were grown on collagen-coated coverslips and in organ culture systems using gelfoam sponge matrices and Millipore filter platforms. The tumor cultured on collagen-coated coverslips initially exhibited the cytologic features of simple uni- and bipolar glial cells and later developed an epithelial-like pattern. Delicate branching of argyrophilic cell processes was demonstrated in Bodian preparations. In organ cultures, the tumor architecture was well preserved for approximately 4 weeks, and mitotic figures were present. Invasion of the sponge foam matrix was seen. Later cultures demonstrated progressive perivascular sclerosis and hyalinization. By electron microscopy, the tumor cells in organ culture systems retained the major fine structural characteristics of the original neoplasm up to 27 daysin vitro. The most striking feature in the late stages was the extensive increase and reduplication of basement membrane material, which appeared related to the increase of collagen fibrils in the extracellular space.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00687629
