ISSN:
1432-069X
Keywords:
Histamine H1-receptor
;
[3H]Mepyramine binding
;
Vascular endothelial cell
;
Guinea pig skin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary In this study, a new procedure for the cultivation of dermal vascular endothelial cells from guinea pig ear lobe was established, and specific binding of [3H]mepyramine to the cultured cell membranes was demonstrated. The vascular endothelial cells were successfully isolated from the dermis of guinea pig ear lobes by the intravenous injection of dispase. Guinea pig serum, as a supplementary component in culture medium, is more suitable for the culture of guinea pig dermal vascular endothelial cell than is fetal calf serum. The [3H]mepyramine binding study indicated the presence of a large number of H1-receptors on the membrane of dermal vascular endothelial cells of guinea pigs, suggesting the important role of endothelial cells in cutaneous reactions to histamine.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00493469
