Electronic Resource
Springer
Histochemistry and cell biology
29 (1972), S. 340-354
ISSN:
1432-119X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary 1. A new method for the light and electron microscopical demonstration of a carboxylesterase is described. Until identified this enzyme is designated as thiolesterase α. 2. The distribution of this enzyme has been studied in 15 mouse organs. The localization of enzyme activity in 11 organs corresponds well with the classical methods employed in the histochemistry of esterases. 3. The main reaction in liver and kidney, as revealed by electron microscopy, is associated with structures of the endoplasmic reticulum. The reaction product appears to be of either vesicular or tubular type and displays an entirely different distribution, as demonstrated with the hitherto existing methods. It is assumed that only a single enzyme is essentially demonstrated. Possibly, it represents an enzyme which is as yet not described in sufficient detail. 4. The tubular and the vesicular type of enzyme reaction is respectively interpreted as a result of distinct functional states of the cells. The possibility of an intermittent synthesis of this enzyme in single cells is considered.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00279816
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