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Glycerol increases the cytochemical detectability of cholesterol in the apical plasma membrane of uterine epithelial cells (1987)

Murphy, C. R. ; Dwarte, D. M.

Springer

Histochemistry and cell biology 87 (1987), S. 7-11

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ISSN: 1432-119X

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary The apical plasma membrane of uterine epithelial cells in the rat has been treated with glycerol before fixation and then examined by freeze-fracture cytochemistry using digitonin and filipin. Many more lesions were produced by both cytochemicals following glycerol treatment than in untreated controls, and we suggest that this indicates an increased detectability of cholesterol. We consider the implications of the findings for the way in which glycerol acts on membranes and propose that glycerol promotes increased binding between cholesterol and the cytochemicals.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00518718

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