ISSN:
1432-2048
Keywords:
Anthocyanin biosynthesis
;
Anthocyanin-3′,5′-methyltransferase
;
Petunia (anthocyanin)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Four S-adenosyl-l-methionine:anthocyanin-3′,5′-O-methyltransferases in flowers of Petunia hybrida were separated using the chromatofocusing technique. Each methyltransferase is controlled by one of the methylation genes Mt1, Mt2, Mf1 or Mf2. Molecular weight, pH-activity optimum, isoelectric point, several kinetic properties and the behaviour in the presence of Mg2+, ethylenediaminetetraacetic acid and S-adenosyl-l-homocysteine of each of the four enzymes were determined. The methylation in vitro of delphinidin 3-(p-coumaroyl)-rutinosido-5-glucoside reflected the accumulation patterns of methylated anthocyanins in vivo and established the regulatory role of methyltransferases in vivo.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392867
