ISSN:
1432-2048
Keywords:
Brassica
;
Pistil specific gene
;
Pollination
;
Self-incompatibility
;
S-locus glycoproteins
;
Transmitting tissue
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In Brassica, self-incompatibility has been correlated with S-locus glycoproteins (SLG) localized at the site of pollen rejection, the stigmatic papillae. In this paper we present immunocytochemical evidence that during development of the pistil SLG proteins accumulate not only in the stigmatic papillae, but also in the transmitting tissue of stigma, style and ovary, along the pathway followed by the pollen tube. This observation was confirmed by the biochemical characterization of stylar and ovarian SLG proteins and by the detection of SLG transcripts in style/ovary tissues by polymerase chain reaction analysis. Thus we demonstrate similarities between the pattern of SLG gene expression in Brassica (sporophytic self-incompatibility system) and that of S products in Nicotiana (gametophytic self-incompatibility system) which may reflect relationships between the self-incompatibility systems. An active role of SLG gene products during the pollen-tube growth in the pistil is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00203663
