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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 32 (1969), S. 181-190 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Summary Merozoites of Babesia ovis in the hemolymph of Rhipicephalus bursa and of Babesia bigemina in the hemolymph of Boophilus microplus and Boophilus decoloratus were tested cytochemically for DNA, RNA, lipids and polysaccharides. The nucleus of the merozoites is Feulgen positive. In incompletely developed merozoites the nucleus is uniformly packed with DNA. In fully developed merozoites the DNA-material is concentrated in the peripheral zone of the nucleus. No Feulgen positive material was detected in the cytoplasm of the merozoites. Abundant amounts of RNA occur in all of the cytoplasm of incompletely developed merozoites. In fully developed merozoites, most of the RNA-material is concentrated in the vicinity of the nucleus, where intensely stained pyroninophilic areas were observed, especially in merozoites of B. ovis. No nucleolar RNA was detected. Some merozoites contain small amounts of lipids. PAS positive material is obviously absent in the merozoites tested in this study.
    Notes: Zusammenfassung An Merozoiten von Babesia ovis in der Hämolymphe von Rhipicephalus bursa und Merozoiten von Babesia bigemina in der Hämolymphe von Boophilus microplus und Boophilus decoloratus wurden cytochemische Tests auf DNS, RNS, Lipide und Polysaccharide durchgeführt. Der Merozoitenkern ist feulgenpositiv. Die den Kern undifferenzierter Merozoiten kompakt ausfüllende DNS konzentriert sich in differenzierten Formen an der Kernperipherie. Im Cytoplasma ist kein feulgenpositives Material nachzuweisen. RNS ist im gesamten Cytoplasma der undifferenzierten Merozoiten reichlich vorhanden. In differenzierten Merozoiten akkumuliert die hauptsächliche Masse der RNS in Kernnähe und bildet besonders bei B. ovis intensiv pyroninophile perinukleäre Konzentrationen. Nukleoläre RNS war nicht nachzuweisen. In einem Teil der Merozoiten sind Lipide in geringer Menge vorhanden. PAS-positives Material (Glykogen) scheint in den untersuchten Formen zu fehlen.
    Type of Medium: Electronic Resource
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