ISSN:
1432-1955
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The nuclear 16S-like rRNA coding regions of two strains of the kinetoplastid flagellateDimastigella trypaniformis Sandon (strain Ulm and strain Glasgow) were sequenced and phylogenetically analyzed. Strain Ulm was isolated from the hindgut contents of the Australian termiteMastotermes darwiniensis Frogatt, whereas strain Glasgow originates from a soil sample in Scotland. After preparation of genomic DNA the 16S-like rRNA coding regions were amplified using polymerase chain reaction (PCR) technology. The amplification products were cloned in a plasmid vector and sequenced according to standard methods. The sequence of the 16S-like rRNA coding region of strain Ulm differs less than 2% from the sequence of strain glasgow, indicating that the two strains are most probably members of one species. Phylogenetic analysis of the sequence data positionedD. trypaniformis Sandon as a deep branching lineage near the root of the kinetoplastid group of flagellates.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00932951
