ISSN:
1617-4623
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The growth of E. coli K12 strains derepressed for N-α-acetyl-L-ornithinase (the argE gene product) is inhibited by 25 mg/ml N-α-acetyl-DL-norvaline. This is due to deacetylation of acetylnorvaline by acetylornithinase yielding norvaline which inhibits growth. A genetically repressible (argR +) strain (514) in which acetylornithinase is repressed is resistant. Thus, acetylnorvaline resistance can be used to select argR + recombinants and transductants from a genetically derepressed (argR -) strain (514-1). ArgR - strains lacking acetylornithinase activity are resistant to acetylnorvaline. Strain 514-1-34 (argR -) which is completely deleted for argE is resistant. Also, of 52 spontaneous acetylnorvaline-resistant mutants derived from strain 514-1, 45 were argE -.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00272178
