ISSN:
1617-4623
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Synthesis of trp mRNA in vitro directed by plasmid DNA carrying the entire trp operon was studied using crude protein extracts (S-100) from Escherichia coli strains carrying the nusA or nusB mutation or both. It was found that the levels of trp mRNA transcribed from the promoter-distal genes (trpCBA) relative to that from the promoter-proximal genes (trpED) was markedly lower with extracts from the nus- mutants than that from the nus + strain. Kinetic experiments suggest that termination of RNA transcripts at intragenic transcriptional barriers is prevented by the nus gene products from allowing efficient experession of the operon.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00330816