ISSN:
1617-4623
Keywords:
Nitrate reductase
;
Fusion pools
;
oxrA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A general method was developed for the isolation of Salmonella thyphimurium LT2 Mu d1–8 (Apr lac) operon fusions in a gene displacing a Tn10 insertion. Random Mu d1–8 fusion pools were prepared to grow phage P22 lysates which transduced chlC::Tn10 to AprTets on fusaric acidampicillin plates. Among these AprTets potential chlC::Mu d1–8 fusions, a simple spot test identified the fusions that were closely linked to the Tn10 insertion in chlC. Out of 68 AprTets isolates 7 chlC::Mu d1–8 fusions with a nitrate-induced Lac+ phenotype were obtained. When oxrA::Tn10 was transduced into these chlC::Mu d1–8 fusions, they became Lac- even in the presence of nitrate, confirming that they were chlC::Mu d1–8 fusions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00430431
