ISSN:
1617-4623
Keywords:
Direct DNA sequencing
;
70 S ribosomes
;
Antibiotic resistance
;
Erythromycin
;
Peptidyl transferase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Experiments designed to establish stable chloroplast transformation require selectable marker genes encoded by the chloroplast genome. The antibiotic lincomycin is a specific inhibitor of chloroplast ribosomal activity and is known to bind to the large ribosomal subunit. We have investigated a defined region of the chloroplast 23 S rRNA genes from four lincomycin resistant Nicotiana plumbaginifolia mutants and from wild-type N. plumbaginifolia. The mutants LR415, LR421 and LR446 have A to G transitions at positions equivalent to the nucleotides 2058 and 2059 in the Escherichia coli 23 S rRNA. The mutant, LR400, possesses a G to A transition at a position corresponding to nucleotide 2032 of the E. coli 23 S rRNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00337724