ISSN:
1617-4623
Keywords:
Mini-P1 plasmid
;
Mini-F plasmid
;
Replication
;
hupA-hupB mutant
;
himA-hip mutant
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Complementation and sequencing analyses revealed that the hopD mutants, which could not support stable maintenance of mini-F plasmids (Niki et al. 1988), had mutations in the hupB gene, and that the hopD410 mutation was an ochre mutation at the 5th Gln position of HU-1. Maintenance and stability of various plasmids, mini-P1 plasmids, mini-F plasmids, and oriC plasmids, were studied in the hupA and hupB mutants (HU mutants), and himA and hip mutants (IHF mutants). Mini-P1 plasmids and mini-F plasmids could not be introduced into the ΔhupA-ΔhupB double deletion mutant. Replication of mini-F plasmids was partially inhibited in the hupB mutants, including the ΔhupB and hopD(hupB) mutants, whereas replication of oriC plasmids was not significantly affected even in the ΔhupA-ΔhupB double deletion mutant. The mini-P1 plasmid was slightly unstable in the himA-hip mutant, whereas the mini-F plasmid was stable.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00260482
