ISSN:
1617-4623
Schlagwort(e):
Brassica
;
Flower
;
Self-incompatibility
;
S locus glycoprotein
;
S locus receptor kinase
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract A new member of the S gene family,SLR3 (S-Locus Related 3), was identified inBrassica oleracea. This gene had a novel pattern of expression compared with previously described members of the family, being expressed in petals, sepals and vegetative apices, in addition to stigmas and anthers. Moreover, use ofSLR3-derived probes in RNA blot and RACE-PCR (rapid amplification of cDNA ends-polymerase chain reaction) experiments has identified transcripts of genes closely related toSLR3 in leaves, cotyledons and, at high levels in developing anthers.SLR3 is not linked to theS locus but is linked to two or three closely related genes. Sequence analysis of theSLR3 gene indicates that it is derived from an ancestral receptor kinase gene that has been modified by a series of deletion events. As a result of these modifications,SLR3 is predicted to encode a secreted glycoprotein lacking both transmembrane and kinase domains. The putativeSLR3 protein differs from the products of most other S gene family members in that several of the highly conserved cysteines have been lost. Within theS gene family, modification of receptor kinase genes by deletion may represent a general mechanism for the generation of genes encoding secreted glycoproteins.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02190796
