ISSN:
1432-0878
Keywords:
Ouabain
;
Tissue culture
;
Mucus
;
Cystic fibrosis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The role of electrolyte transport in the metabolism of mucosubstance has been investigated using cell cultures derived from rat submaxillary gland. When added to the culture medium at a concentration of 1 mM, ouabain, an inhibitor of active alkali metal ion transport through cell membranes, causes extensive cytoplasmic vesiculation suggestive of an accumulation of secretory materials within the cell; the rough ER and cytoplasmic polysomes are not affected. Isotopic experiments support these conclusions by demonstrating that ouabain inhibits the secretion of radioactive mucosubstances by the cells; comparative studies with ouabain and puromycin (2×10−4M) indicate that the inhibitory effect of ouabain is not due to an inhibition of protein biosynthesis. A relationship between secretion and alkali metal ion movement is suggested which may be of significance in connection with the genetic disease, cystic fibrosis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00324807
