ISSN:
1432-0878
Keywords:
Pancreatic polypeptide
;
Endocrine human pancreas
;
Histochemistry
;
Immunohistochemistry
;
Electron microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The consecutive semithin — thin section method was employed to identify pancreatic-polypeptide-containing cells in the human pancreas. The immunocytochemical staining of semithin sections with the peroxidase-antiperoxidase complex allowed a precise ultrastructural description of the immunoreactive cells on the adjacent thin section. Two populations of small granulated cells could be distinguished with this technique: one group is formed by cells whose secretory granules with a mean diameter of 141 nm show specific immunoperoxidase staining with anti-PP serum, while the other group consists of cells whose secretory granules measuring 118 nm in diameter show no immunoreactivity to anti-PP serum. The former cells which will be designated as PP-cells, are not identical with the D1-cells of the revised Wiesbaden classification of endocrine cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00220668