ISSN:
1432-0878
Keywords:
Key words: Retina
;
Glutamate transporter subtypes (GLT-1
;
EAAC-1
;
GLAST-1)
;
Expression
;
Anti-peptide antibodies
;
Immunohistochemistry
;
Glutamatergic pathways
;
Rat (Wistar
;
Brown Norway)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract. The immunocytochemical distribution of the three excitatory amino acid transporter subtypes GLT-1, GLAST-1 and EAAC-1 was studied in the rat retina using antibodies raised against synthetic peptides corresponding to the C-terminus of each transporter subtype (Rothstein et al. 1994). A comparative immunoblot analysis of rat cortex, cerebellum and retina membrane proteins suggested the following rank order of glutamate transporter subtype expression in retina: GLAST-1≥EAAC-1〉GLT-1. GLAST-1 immunoreactivity was seen in Müller cells and astrocytes. EAAC-1 was found in horizontal cells, in amacrine and displaced amacrine cells, and in ganglion cells. A minority of bipolar cells also expressed EAAC-1. GLT-1 was preferentially expressed by different types of bipolar cells; however, it was also found in some amacrine cells. The functional role of this differential distribution of glutamate transporters in the retina is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004410050702
