ISSN:
1476-5535
Keywords:
supercoiling
;
enzymes
;
acetone
;
butanol
;
Clostridium acetobutylicum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Cells ofClostridium acetobutylicum treated with novobiocin, a DNA gyrase inhibitor, produced higher butyrate levels and lower solvent levels with acetone being the most affected. Seven enzyme activities involved in acid and solvent production were analyzed. Among them, only CoA transferase, required for acetone formation and acid uptake, experienced a significant decrease in activity. As inEscherichia coli andBacillus subtilis, DNA fromC. acetobutylicum became less negatively supercoiled in the early stationary phase (solventogenic stage), as shown by analysis of linking number of a reporter plasmid by agarose gel electrophoresis in the presence of chloroquine.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01570115
