ISSN:
1591-9528
Keywords:
Isolated rat kidney
;
Na net transport
;
Pluronic-F-108®
;
Electron microscopy
;
Oxygen deficiency
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Employing a non-ionic detergent as a colloidosmotically active substance 10 fully isolated rat kidneys were perfused for 60 min. A constant glomerular filtration rate (GFR) of 1.0 ml × g−1 × min−1 and a mean Na+-net transport of about 92 μmol × g−1 × min−1 was obtained. The mean O2-consumption of 0.17 ml × g−1 × min−1 was rather high. However, addition of the decoupling agent 2,4-dinitrophenol led to an increase of the O2-consumption by 98%, thus speaking against spontaneous uncoupling. Glomerula, proximal and distal convoluted tubules of kidneys perfused with plasmaexpander-solutions were studied electronmicroscopically. The most striking morphological deviations were found in the proximal tubules of the Haemaccel®-perfused kidneys. In the Pluronic®-kidneys significantly less morphological alterations were found. These morphological changes were of a type which was previously described by other investigators after periods of hypoxia.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02045828
